Shreenivas Kumar Singh

Hardworking and passionate post-doctoral researcher with a demonstrated record of research and keen interest in teaching to bridge the gap between academic and research filed. Ability to design and execute long term molecular biology research in laboratory.

• CSIR JRF-NET, 12/2013, 330513, 46
• DBT JRF, 2014, 105140, Group A
• ASRB NET, 2014, 1200100250

• Pathogen related- Handling of pathogenic fungal strain Ascochyta rabie, Bipolaris sorokiniana and Piriformospora indica. PEG mediated genome editing, Agrobacterium tumefaciens mediated transformation (ATMT) of A. rabiei for gene overexpression and localization and RNAi based gene silencing.
• Genomics related- PCR technique, gene cloning (restriction digestion, blunt end cloning, Gateway based cloning), DNA and RNA purification from plant and fungal tissues.
• Yeast strain- Expertise in handling yeast strains and molecular work like Yeast-2-Hybrid, Yeast-3-Hybrid, Yeast-1-Hybrid.
• Proteomics work- Protein expression and purification, western blot, Size exclusion chromatography, Pull-Down assay, Electrophoretic mobility shift assay.
• Gene localization work- Agrobacterium transformation, transient expression in Nicotiana benthamiana, Bimolecular fluorescence complementation (Bi-FC) Dual-Luciferase assay, FRET (for in vivo protein-protein interaction) and Confocal laser scanning microscopy.

• García, P.; Singh, S.; Graciet, E. New Insights into the Connections between Flooding/Hypoxia Response and Plant Defenses against Pathogens. Plants 2024, 13, 2176. https://doi.org/10.3390/plants13162176
• Shreenivas Kumar Singh, Ankita Shree, Sandhya Verma, Kunal Singh, Kamal Kumar, Vikas Srivastava, Ritu Singh, Samiksha Saxena, Agam Prasad Singh, Ashutosh Pandey, Praveen Kumar Verma, The nuclear effector ArPEC25 from the necrotrophic fungus Ascochyta rabiei targets the chickpea transcription factor CaβLIM1a and negatively modulates lignin biosynthesis, increasing host susceptibility, The Plant Cell, https://doi.org/10.1093/plcell/koac372
• Manisha Sinha, Ankita Shree, Kunal Singh, Kamal Kumar, Shreenivas Kumar Singh, Vimlesh Kumar, Praveen Kumar Verma, Modulation of fungal virulence through CRZ1 regulated F-BAR-dependent actin remodeling and endocytosis in chickpea infecting phytopathogen Ascochyta rabiei, Plos Genetics, https://doi.org/10.1371/journal.pgen.1009137
• Maurya, R., Singh, Y., Sinha, M., Singh, K., Mishra, P., Singh, S.K., Verma, S., Prabha, K., Kumar, K., and Verma, P.K., Transcript profiling reveals potential regulators for oxidative stress response of a necrotrophic chickpea pathogen Ascochyta rabiei, 3 Biotech, https://doi.org/10.1007/s13205-020-2107-8
• S.K. Singh, S. Verma, P.K. Verma, Genetically engineered crops against bacterial and fungal diseases: a war of attrition, Elsevier (Book chapter)
• Ravi Ranjan Saxesena, Shreenivas Kumar Singh, Praveen Kumar Verma, Transcription factors: master regulators of disease resistance in crop plants, Academic Press, Plant Transcription Factors (Book Chapter), https://doi.org/10.1016/B978-0-323-90613-5.00009-1