Emmet O'Shea
Summary
Detail-oriented Biochemistry graduate with hands-on experience in molecular biology and over 700 hours of practical laboratory work. Skilled in DNA-based techniques, experimental design, and data analysis while working within regulated environments. Strong understanding of protein-protein interactions, with a focus on generating reproducible high-quality data. Seeking an entry level role in biotechnology or pharmaceutical R&D to contribute to experimental research and development.
Overview
4
4
years of professional experience
Work history
Customer Assistant
O'Leary's Daybreak Tower
2022.01 - 2026.04
- Delivered efficient and friendly customer service in a fast-paced environment.
- Managed stock control, ensuring efficient inventory tracking.
- Resolved customer queries and issues professionally under pressure.
Protein-Protein Interactions (SMAUG1)
University College Cork
2025.01 - 2025.04
- Investigated the role of SMAUG1's C-terminal region in mediating protein-protein interactions involved in cellular signalling and transcriptional regulation.
- Identified overlapping PDZ and 14-3-3 binding motifs, suggesting possible interactions with structural and signalling proteins.
- Developed a strong theoretical understanding of NanoBRET (Bioluminescent Resonance Energy Transfer) methodology which utilises NanoLuc luciferase and HaloTag fluorescent ligands to detect interactions in live cells.
- Understood the application of NanoBRET in detecting weak and transient interactions not readily observable through standard assays.
- Contributed to understanding SMAUG1 dysregulation in disease contexts, such as cancer and neurodegenerative disorders, highlighting potential therapeutic targets.
- Generated and analysed experimental data, identifying interaction trends and supporting evidence based conclusions.
- Maintained accurate laboratory records.
- Worked independently in compliance with strict GLP and health and safety standards.
Education
BSc Biochemistry | Level 8 Qualification -
University College Cork
09/2021 - 05/2025
Leaving Certificate -
Scoil Mhuire Gan Smal
09/2015 - 05/2021
Skills
Analytical Skills
- SDS-PAGE
- pH measurement & calibration
- UV/Vis Spectrophotometry
- Agarose Gel Electrophoresis
- High-performance Liquid Chromatography (HPLC)
Molecular Biology Techniques
- Bacterial Transformation
- Mini-Preparations
- Polymerase Chain Reaction (PCR)
- DNA Isolation & Purification
- DNASequencing
Cell & Protein Analysis
- Cell Culturing
- Transfection
- Western Blotting
Laboratory & Data Skills
- Good Laboratory Practice (GLP)
- Standard Operating Procedure (SOP) adherence
- Experimental design and optimisation
- Data analysis and interpretation
- Sample handling & tracking
Additional Skills
- Microsoft Office (Word, PowerPoint, Excel)
- Strong time management and organisation abilities
- Scientific writing and presentation
- Effective teamwork and communication
Training & Qualifications
- ~700 hours laboratory experience (Accumulated over 4 years)
- HAACP & Manual Handling
- First Aid Training
References
- Dr. Kellie Dean (School of Biochemistry and Cell Biology) k.dean@ucc.ie | +353 (0)21 420 5421
Timeline
Protein-Protein Interactions (SMAUG1)
University College Cork
2025.01 - 2025.04
Customer Assistant
O'Leary's Daybreak Tower
2022.01 - 2026.04
BSc Biochemistry | Level 8 Qualification -
University College Cork
09/2021 - 05/2025
Leaving Certificate -
Scoil Mhuire Gan Smal
09/2015 - 05/2021