Aparna KG

• Perform routine maintenance, assay calibrations and verification of laboratory instruments.
• Assist in assay validation and verification activities, supporting method performance and documentation.
• Recorded precise internal and external QC data to ensure full traceability and compliance with ISO 15189 standards.
• Routinely used IAMQC Infinity (cloud QC platform) for daily trend analysis, rule-based QC alerts and multi-instrument QC review.
• Troubleshot IQC failures using physical and analytical testing methods.
• Documented experimental procedures and results to maintain comprehensive records.
• Managed inventory effectively to guarantee availability of essential supplies.
• LIMS: Sample reception and tracking, non-conformance documentation, supporting sample identification and workflow.
• Routinely monitored laboratory temperature systems, recorded deviations and carried out appropriate corrective actions.

• Co-ordinating and management of sample pulls. Management of freezers and allocating exact locations as per relevant BOT, SOP, SPECs physically and on eLIMS.
• High competency in laboratory methods and procedures to support in-process and release testing of biotechnology products.
• Review of laboratory test results
• Aliquoting of samples as per associated BOT and shipment of samples to internal and external sites for analysis.
• Reference material inventory management. Maintenance of accurate and legible documentation for all activities on real-time.
• Adhering to all company standards in safety, housekeeping and quality. Maintaining 6S within laboratories.
• Notifying management of any discrepancies and deviations.
• Freezer temperature data management and alarm responder.
• Collaborating with partner sites, clients, suppliers, and fellow associates to define and improve quality standards and maintain expectations.
• Study work processes, measurements, and performance metrics to identify improvement areas.

• Performed cell culture and maintained three cell lines – HeLa, Wharton's Jelly stem cells and PMSCs.
• Assessment of cell viability through MTT cell proliferation assay. ELISA for detection and quantification of HIF-1α, VEGF, IL-6.
• Gene expression was also studied using Real time PCR.
• Currently working for book-review chapter on Prenatal Mesenchymal Stromal Cell as potential therapeutic tool, is soon to be published.

• Worked with pharmaceutical analysis of raw materials and finished products.
• Operations of the following Instruments:

– Fourier-transform infrared spectroscopy (FTIR) analysis

– Fluorescence Spectrometer LCMS/MS

– Gas Chromatography and Hydrogen flame ionization detector (Analysis of Cholesterol, and Fats)

– Inductively Coupled Plasma Optical Emission Spectrometry (ICP- OES) and Inductively Coupled Plasma Atomic Emission Spectroscopy (ICP-AES)