Anish Nair
Ballina,
Summary
- Biotechnologist with 5+ years in precision oncology, specializing in targeted therapy curation and genomic variant analysis
- Proficient in RT-PCR, SDS-PAGE, flow cytometry, diverse microscopy, and GMP-regulated projects
- Skilled in managing cell cultures, biosafety testing, and ensuring regulatory compliance
- Expert in collaborative research, and biocuration at PierianDX for clinical genomics
- Track record in project management, team training, and QA cycles
- Eager to lead impactful advancements in the biopharma industry
Overview
7
7
years of professional experience
1
1
Certification
Work History
In-Vitro Bioassay Analyst
Charles River Laboratories
Ballina
10.2023 - Current
- Spearheaded GMP-regulated molecular assays: RT-PCR, dPCR, and ddPCR to detect residual DNA, showcasing precision and compliance expertise
- Managed Mammalian Cell Cultures with meticulous adherence to GMP standards, ensuring precision and excellence
- Played a pivotal role in method qualifications, PSQs, and technology transfers
- Conducted QC and Biosafety testing of samples, ensuring regulatory compliance
- Adept in DNA/RNA isolation using Kingfisher technology
- Draft and implement Standard Operating Procedures (SOPs), rigorously adhering to these protocols, and ensuring absolute compliance with GMP regulations
- Instrumental in data review, significantly impacting project outcomes
- Led lab management responsibilities, including monthly equipment checks for GMP compliance
- Acquainted with ICH-GCP guidelines for testing
- Performing test method procedures with precision, executing protocols, and generating comprehensive reports within tight time constraints, demonstrating a commitment to efficiency and accuracy
- Proficient in LIMS, enhancing data management efficiency
- Collaborate seamlessly in laboratory investigations as needed, while proactively crafting meticulous risk assessments for both laboratory operations and test items as mandated
- Serving as the point of contact between the calibration and molecular department, ensuring equipment maintenance in compliance with standards
Student Researcher
Dublin City University
Dublin
03.2023 - 08.2023
- Collaborated on a sophisticated research project in Dr
- Michael Freeley's lab, investigating Jurkat cell behavior
- Employed techniques including proliferation assays and microscopic analysis with ImageJ to scrutinize proliferation and cell size in three distinct Jurkat cell lines
- Executed intricate cell transfection procedures using electroporation, lipofectamine, and TransIT methodologies
- Applied flow cytometric analysis and western blotting to discern and characterize specific markers on Jurkat cells
- Performed laboratory research in strict adherence to Good Laboratory Practice (GLP) guidelines, emphasizing precise documentation and rigorous protocol compliance to uphold the integrity and reliability of scientific data.
Biological Data Analyst (Biocurator)
PierianDX India Pvt. Ltd.
Pune
07.2020 - 07.2022
- Expertise in Clinical Genomics, Precision Medicine, and Variant Analysis
- A clear understanding of FDA/EMA drug labels, disease guidelines (NCCN, ASCO, ESMO)
- Gathering and synthesizing data from scientific literature published by clinical and human genome research groups
- Documenting the impact of genomic variants on disease, utilizing controlled vocabularies, ontologies, and established guidelines
- Employed advanced web tools, including mutalyzer and transvar, to analyze and interpret genomic alterations
- Competent in navigating genome ontology databases, including clinvar, gnomad, COSMIC, and genbank for data curation
- Conducting Quality Assurance (QA) cycles to ensure data integrity
- Created training initiatives aligned with standard GDP to enhance the onboarding and development of new staff members
- Led the introduction of an improved screening process, leading to a 30% decrease in screening duration without compromising accuracy and precision
- Consistently achieving and surpassing productivity goals, I upheld a 97% on-time completion rate in annotation, actively enhancing our operational performance
- Efficiently managed multiple projects and collaborating with interdisciplinary teams.
Associate Scientist
Cyca Onco Solutions Pvt.ltd
Pune
07.2019 - 07.2020
- Expertise in managing cell lines and conducting molecular biology experiments
- Adept in aseptic techniques for culturing and cloning bacteria
- Experienced in protein isolation and purification processes
- Skilled in performing chemical conjugation reactions
- Proficient in Molecular Dynamics Simulation using Gromacs
- Advanced skillset in CRISPR/Cas9-mediated gene delivery, underlining a deep understanding and practical application of cutting-edge genetic manipulation techniques
- Demonstrated expertise in employing GraphPad Prism for in-depth statistical analysis, contributing to accurate and strategic decision-making processes
- Adherence to GLPs with a track record of generating proof of concept for designed products.
Project Assistant
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University
Pune
05.2017 - 03.2019
- Coordinating and supporting active laboratory projects
- Supervising and training students in project-related tasks
- Formulating experimental designs to investigate project objectives and scope
- Executing diverse cell and molecular biological experiments, such as PCRs, viability assessments, potency, and migration assays
- Effectively administered the chemical inventory system and optimized procurement procedures through the establishment and management of comprehensive laboratory supply lists.
Education
Master of Science - Diagnostics And Precision Medic
Dublin City University
Dublin, Ireland09-2023
Master of Science - Biotechnology
Pune University
Pune, India01-2017
Skills
- RT-PCR, dPCR and ddPCR
- Protein isolation and purification (Affinity and Size exclusion)
- SDS-PAGE and Western Blotting
- ELISA
- Molecular cloning
- Gene profiling and analysis
- Bioinformatics Tools
- Mammalian cell culture
- Confocal and Electron Microscopy
- Zymography
- Flow cytometry (FACS)
- Transfection
- Cell based assays
- Biostatistics
Certification
- Python fundamentals for beginners (Great Learning offered basic python course)
- Introduction to Genomic Technologies (Johns Hopkins University)
- ISO 14971:2019 - Risk Management Application on Medical Devices (Alison - Empower Yourself)
Publications
- Macro- and micro-nutrient–based multiplex stress conditions modulate in vitro tumorigenesis and aggressive behavior of breast cancer spheroids (Springer)
Timeline
In-Vitro Bioassay Analyst
Charles River Laboratories
10.2023 - Current
Student Researcher
Dublin City University
03.2023 - 08.2023
Biological Data Analyst (Biocurator)
PierianDX India Pvt. Ltd.
07.2020 - 07.2022
Associate Scientist
Cyca Onco Solutions Pvt.ltd
07.2019 - 07.2020
Project Assistant
Institute of Bioinformatics and Biotechnology, Savitribai Phule Pune University
05.2017 - 03.2019
Master of Science - Diagnostics And Precision Medic
Dublin City University
Master of Science - Biotechnology
Pune University
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