Alireza Ahadiabhari
Edmonton
Summary
M.Sc. graduate in Anatomy, Physiology, and Pharmacology with extensive experience in molecular biology techniques, including DNA/RNA extraction, PCR, qRT-PCR, and gene expression analysis. Skilled in experimental design, data analysis, and troubleshooting. Seeking a Ph.D. Position to contribute to cutting-edge research while expanding expertise in molecular biology. Highly motivated, detail-oriented, and eager to collaborate in an interdisciplinary research environment.
Overview
6
6
years of professional experience
Work History
Lab Technician
University of Saskatchewan
09.2022 - 12.2024
- Conducted molecular biology experiments, including RNA/DNA extraction, PCR, qRT-PCR, cloning, SDS-PAGE, agarose gel electrophoresis, Genotyping, Western blotting, ELISA, and Immunofluorescence.
- Performed gene knock-down and knock-out experiments using shRNA and siRNA lentiviral infection and CRISPR-Cas9.
- Authored and maintained standard operating procedures (SOPs) and non-conformities documents.
- Collaborated with Principal Investigators to design and set up experiments, optimize protocols, and analyze data.
- Reviewed and interpreted scientific literature to implement new techniques and improve experimental outcomes.
- Performed data analysis using software like SPSS, R, and GraphPad Prism, interpreting results, and contributing to the preparation of research reports, papers, and presentations and contributing to manuscript preparation and grant proposals.
Teaching Assistant
University of Saskatchewan
01.2022 - 04.2022
- Prepared and demonstrated laboratory material
- Setting up experiments, marking, invigilating examinations, delivering briefings, supervising field trips
- hosted virtual meetings via WebEx/Zoom, and conferred with the Supervisor in charge as required by the assignment.
Teaching Assistant
University of Saskatchewan
09.2021 - 12.2021
- Involved in laboratory techniques course by assisting students in laboratory techniques involving PCR, Gel electrophoresis, Spectrophotometers, and Chromatography.
- Provided lab quizzes and grades on the material.
- Supervised and evaluated students' laboratory work
- Advised students on academic plans and course selection.
- Marking assignments and exams, and providing feedback to students'.
Research Assistant
Shahid Beheshti University of Medical Science
12.2018 - 07.2021
- Conducted research on miRNA expression profiles in gastric cancer progression using patient-derived clinical samples.
- Performed total RNA extraction from tissue and blood samples using the TRIzol-based method.
- Designed and optimized qRT-PCR assays for miRNA expression analysis.
- Analyzed qRT-PCR data to identify differentially expressed miRNAs associated with gastric cancer stages.
- Worked closely with clinicians and pathologists to correlate molecular data with clinical outcomes.
- Maintained accurate and detailed laboratory records, ensuring reproducibility of experiments.
- Assist in manuscript preparation and presentation of findings at scientific conferences.
Education
Master of Science - Anatomy, Physiology, And Pharmacology
University of Saskatchewan
Saskatoon, Saskatchewan12-2023
Master of Science - Human Genetics
Shahid Beheshti University of Medical Sciences
Tehran, Iran07-2018
Bachelor of Science - Cellular And Molecular Biology
Islamic Azad University
Tehran, Iran01-2016
Skills
- Tissue Culture
- DNA/RNA extractions
- PCR, qRT-PCR
- SDS-PAGE
- Gel Electrophoresis
- Genotyping
- Western Blot
- ELISA
- Immunofluorescence Staining
- Microsoft Office, SPSS, ImageJ, R, and CorelDRAW
Accomplishments
- Alireza Ahadi, A systematic review of microRNAs as potential biomarkers for diagnosis and prognosis of gastric cancer, Immunogenetics, 12/10/2020, https://doi.org/10.1007/s00251-020-01201-6.
- Alireza Ahadi, The significance of microRNA deregulation in colorectal cancer development and the clinical uses as a diagnostic and prognostic biomarker and therapeutic agent, Non-coding RNA Research, 08/23/2020, DOI: https://doi.org/10.1016/j.ncrna.2020.08.003.
- Alireza Ahadi, Functional roles of lncRNAs in the pathogenesis and progression of cancer, Genes & Diseases, 04/21/2020, DOI: 10.1016/j.gendis.2020.04.009.
- Alireza Ahadi, Dysregulation of miRNAs as a signature for diagnosis and prognosis of gastric cancer and their involvement in the mechanism underlying gastric carcinogenesis and progression, IUBMB Life, 72(5): 884-898, 02/08/2020, DOI: 10.1002/iub.2259.
- Alireza Ahadi, Marzieh Sadat Safavi, miR-335-5p has an important role in the progression of gastric cancer by down-regulation of CEACAM5, Meta Gene, 19: 65-68, 02/01/2019, DOI: https://doi.org/10.1016/j.mgene.2018.10.013.
- Zahra Fazeli, Alireza Ahadi, Mir D Omrani, Sayyed MH Ghaderian, 2018, Efficiency of mesenchymal stem cells in treatment of urinary incontinence: a systematic review on animal models, Regenerative medicine, 14(1), 12/17/2018, DOI: 10.2217/rme-2018- 0040.
- Alireza Ahadi, Marzieh Sadat Safavi, Mahboobeh Faramin Lashkarian, A role of telomerase in cancer progression and therapeutic program for anti-telomerase approach and future objectives, Health Biotechnology and Biopharma Journal, 2(2): 1-13,09/09/2018, http://www.healthbiotechpharm.org/issue6.htm.
- Keramatinia A, Ahadi A, Akbari ME, Mohseny M, Mosavi Jarahi A, BahadoriMonfared A, Hashemi M, Moradi A, Mehrvar N, Kazemi E, Movafagh A, The roles of DNA epigenetics and clinical significance in Chronic Myeloid Leukemia: a review, Cellular and Molecular Biology, 64(9): 58-63, 06/30/2018, PMID:30030958, https://pubmed.ncbi.nlm.nih.gov/30030958/.
- Larki P, Ahadi A, Zare A, Tarighi S, Zaheri M, Souri M, Zali MR, Omrani MD, Up- Regulation of miR-21, miR-25, miR-93, and miR-106b in Gastric Cancer, Iranian Biomedical Journal, 22(6): 367-373, 05/2018, DOI: 10.29252/.22.6.367.
- Keramatinia A, Ahadi A, Akbari ME, Mohseny M, Jarahi AM, Mehrvar N, Mansouri N, Tabatabaei SAM, Movafagh A, Genomic Profiling of Chronic Myelogenous Leukemia: Basic and Clinical Approach, Journal of Cancer Prevention, 22(2): 74-81, 06/30/2017, DOI: 10.15430/JCP.2017.22.2.74.
CONFERENCE PRESENTATIONS
- Canadian Society for Molecular Biosciences - University of Ottawa - 08/2023
- 30th Annual Life & Health Sciences Research Expo, Health Sciences , University of Saskatchewan - 04/2023
References
- Dr. Scott Widenmaier : scott.widenmaier@usask.ca/(+1) 306-966-8320
- Dr. Anand Krishnan : anand.krishnan@usask.ca/(+1) 306-655-8711
- Dr. Abbas Akhavan Sepahi : abbas_akhavansepahi@yahoo.com/(+98) 227-16582
Timeline
Lab Technician
University of Saskatchewan
09.2022 - 12.2024
Teaching Assistant
University of Saskatchewan
01.2022 - 04.2022
Teaching Assistant
University of Saskatchewan
09.2021 - 12.2021
Research Assistant
Shahid Beheshti University of Medical Science
12.2018 - 07.2021
Master of Science - Anatomy, Physiology, And Pharmacology
University of Saskatchewan
Master of Science - Human Genetics
Shahid Beheshti University of Medical Sciences
Bachelor of Science - Cellular And Molecular Biology
Islamic Azad University
Similar Profiles
Trenna KriegerTrenna Krieger
Nursing Clinical Educator at University of SaskatchewanNursing Clinical Educator at University of Saskatchewan
Gagan Devaiah KolimaduGagan Devaiah Kolimadu
Graduate Research Assistant at University of SaskatchewanGraduate Research Assistant at University of Saskatchewan
Bright BoamahBright Boamah
Research Assistant at University of SaskatchewanResearch Assistant at University of Saskatchewan
Kartik TiwariKartik Tiwari
Student Crew at University of SaskatchewanStudent Crew at University of Saskatchewan
Simran ChohanSimran Chohan
Associate at Walmart Supercentre CapilanoAssociate at Walmart Supercentre Capilano